Mutants of Coprinus lagopus selected for resistance to 2-Deoxy-D-glucose

The glucose analogue 2-deoxy-D-glucose seriously inhibits the growth of Coprinus lagopus. Following treatment with iV-methyl-iV'-nitro-i^nitrosoguanidine 388 resistant mutants were isolated. It is shown that the mutants isolated are probably allelic; they were phenotypically similar and no complementation was observed. The mutants were pleiotropic in the sense that although they were initially selected only for resistance to 2-deoxy-D-glucose they were found to be cross-resistant to both of the related analogues, sorbose and glucosamine. Furthermore, the mutants were unable to utilize fructose as a sole carbon source. I t is demonstrated that the inability to utilize fructose results from a defect in sugar transport. The gene symbol ftr is proposed for this cistron and it is shown that though the gene is quite closely linked to its own centromere, it is unlinked to centromere markers of the six known linkage groups.